Method of producing heterocyclic derivatives

ABSTRACT

Heterocyclic derivatives of the formula (I)                    
     wherein each symbol is as defined in the specification, pharmaceutically acceptable salts thereof and method of producing them. Pharmaceutical compositions containing the heterocyclic derivative or a pharmaceutically acceptable salt thereof, particularly, ACAT inhibitors and lipoperoxidation inhibitors. The heterocyclic derivatives and pharmaceutically acceptable salts thereof of the present invention show superior ACAT inhibitory activity and lipoperoxidation inhibitory activity, and are useful as ACAT inhibitors and hyperlipemia inhibitors. To be specific, they are useful for the prevention and treatment of arteriosclerotic lesions of arteriosclerosis, hyperlipemia and diabetes, as well as ischemic diseases of brain, heart and the like.

CROSS REFERENCE TO RELATED APPLICATIONS

This patent application is a continuation of U.S. patent application Ser. No. 09/283,525, filed Apr. 1, 1999, now abandoned, which is a divisional of U.S. patent application Ser. No. 08/809,242, filed Mar. 19, 1997, now U.S. Pat. No. 5,990,150, which is the U.S. national phase of PCT International Application No. PCT/JP95/01873, filed Sep. 20, 1995.

TECHNICAL FIELD

The present invention relates to novel heterocyclic-derivatives, a method of production thereof and pharmaceutical use thereof. More particularly, the present invention relates to novel heterocyclic derivatives having an indoline ring or tetrahydroquinoline ring, a method of production thereof and pharmaceutical use thereof, specifically acyl-CoA:cholesterol acyltransferase (hereinafter ACAT) inhibitors and lipoperoxidation inhibitors.

BACKGROUND ART

It is a well-known fact that arteriosclerosis is an extremely important factor causing various circulatory diseases, and active studies have been undertaken in an attempt to achieve suppression of the evolution of arterial sclerosis or regression thereof. In particular, although the usefulness of a pharmaceutical agent which reduces cholesterol in blood or arterial walls has been acknowledged, an ideal pharmaceutical agent exhibiting positive clinical effects while causing less side-effects has not been realized.

In recent years, it has been clarified that cholesterol accumulated in arterial walls in the ester form thereof significantly evolves arteriosclerosis. A decrease in cholesterol level in blood leads to the reduction of accumulation of cholesterol ester in arterial walls, and is effective for the suppression of evolution of arteriosclerosis and regression thereof.

Cholesterol in food is esterified in mucous membrane of small intestine, and taken into blood as chylomicron. ACAT is known to play an important role in the generation of cholesterol ester in mucous membrane of small intestine. Thus, if esterification of cholesterol can be suppressed by inhibiting ACAT in mucous membrane of small intestine, absorption of cholesterol by mucous membrane and into blood can be presumably prevented to ultimately result in lower cholesterol level in blood.

In arterial walls, ACAT esterifies cholesterol and causes accumulation of cholesterol ester. Inhibition of ACAT in arterial walls is expected to effectively suppress accumulation of cholesterol ester.

From the foregoing, it is concluded that an ACAT inhibitor will make an effective pharmaceutical agent for hyperlipemia and arteriosclerosis, as a result of suppression of absorption of cholesterol in small intestine and accumulation of cholesterol in arterial walls.

Conventionally, for example, there have been reported, as such ACAT inhibitors, amide and urea derivatives [J. Med. Chem., 29: 1131 (1986), Japanese Patent Unexamined Publication Nos. 117651/1990, 7259/1991, 32666/1993 and 327564/1992].

However, creation and pharmacological studies of these compounds have been far from sufficient.

Meanwhile, peroxidation of low density lipoprotein (LDL) is also highly responsible for accumulation of cholesterol ester in arterial walls. In addition, it is known that peroxidation of lipids in a living body is deeply concerned with the onset of arteriosclerosis and cerebrovascular and cardiovascular ischemic diseases.

Accordingly, a compound having both ACAT inhibitory activity and lipoperoxidation inhibitory activity is highly useful as a pharmaceutical product, since it effectively reduces accumulation of cholesterol ester in arterial walls and inhibits lipoperoxidation in the living body, thereby preventing and treating various vascular diseases caused thereby.

It is therefore an object of the present invention to provide a compound having ACAT inhibitory activity and lipoperoxidation inhibitory activity, a method for production thereof and pharmaceutical use thereof, particularly as an ACAT inhibitor and lipoperoxidation inhibitor.

DISCLOSURE OF THE INVENTION

The present inventors have conducted intensive studies with the aim of accomplishing the above-mentioned object and found that a certain heterocyclic derivative having an indoline ring or tetrahydroquinoline ring has lipoperoxidation inhibitory activity in addition to strong ACAT inhibitory activity, and that said compound has strong anti-hyperlipemia effect and anti-arteriosclerosis effect, which resulted in the completion of the invention.

Thus, the present invention relates to a heterocyclic derivative of the formula (I)

wherein

one of R¹, R², R³ and R⁴ is a group of the formula —NHCO—R⁶ wherein R⁶ is optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl, optionally substituted aryl, optionally substituted arylalkyl, optionally substituted heterocycle, optionally substituted heterocyclic alkyl, —R^(A)SO₃A, —R^(B)PO₃B where R^(A) and R^(B) are each alkylene and A and B are each alkali metal or hydrogen atom, —NR⁷R⁸ where R⁷ is optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl, optionally substituted aryl or optionally substituted arylalkyl and R⁸ is hydrogen atom or lower alkyl, or —R⁹—OCOR¹⁰ where R⁹ is alkylene and R¹⁰ is optionally substituted alkyl, optionally substituted heterocycle or optionally substituted heterocyclic alkyl, and the remaining three may be the same or different and each is independently a hydrogen atom, a lower alkyl or a lower alkoxy;

R⁵ is an optionally substituted alkyl, an optionally substituted cycloalkyl, an optionally substituted cycloalkylalkyl, an optionally substituted aryl, an optionally substituted arylalkyl, an optionally substituted heterocycle, an optionally substituted heterocyclic alkyl, an alkenyl, an alkynyl, a dialkylaminoacyloxyalkyl, —R^(D)SO₃D or —R^(E)PO₃E where R^(D) and R^(E) are each alkylene and D and E are each alkali metal or hydrogen atom, provided that when R⁴ is —NHCO—R⁶, R⁵ and R⁶ optionally combinedly form a ring; and

m is 1 or 2,

[hereinafter this compound is also referred to as Compound (I)] and a pharmaceutically acceptable salt thereof.

The present invention also relates to a method for producing the above-mentioned heterocyclic derivative or a pharmaceutically acceptable salt thereof, which comprises a step of

{circle around (1)} reacting an amine of the formula (II)

wherein R¹¹, R¹² and R¹³ may be the same or different and each is independently hydrogen atom, lower alkyl or lower alkoxy, and R⁵ and m are as defined above [hereinafter also referred to as Compound (II)], and an isocyanate of the formula (III)

R⁷NCO  (III)

wherein R⁷ is as defined above [hereinafter also referred to as Compound (III)];

{circle around (2)} reacting Compound (II) and a halogen compound of the formula (IV)

R⁶—COX  (IV)

wherein X is halogen atom and R⁶ is as defined above [hereinafter also referred to as Compound (IV)];

{circle around (3)} reacting Compound (II) and a carboxylic acid of the formula (V)

R⁶′COOH  (V)

wherein R⁶′ is optionally substituted alkyl, optionally substituted cycloalkyl, optionally substituted cycloalkylalkyl, optionally substituted aryl, optionally substituted arylalkyl, optionally substituted heterocycle or optionally substituted heterocyclic alkyl [hereinafter also referred to as Compound (V)] or a reactive derivative thereof;

{circle around (4)} reacting an isocyanate of the formula (VI)

wherein R⁵, R¹¹, R¹², R¹³ and m are as defined above [hereinafter also referred to as Compound (VI)], and an amine of the formula (VII)

HNR⁷R⁸  (VII)

wherein R⁷ and R⁸ are as defined above [hereinafter also referred to as Compound (VII)]; or

{circle around (5)} reacting a compound of the formula (VIII)

wherein R¹, R², R³, R⁴ and m are as defined above [hereinafter also referred to as Compound (VIII)], and a compound of the formula (IX)

R⁵X  (IX)

wherein R⁵ and X are as defined above [hereinafter also referred to as Compound (IX)].

The present invention also relates to pharmaceutical compositions, ACAT inhibitors and lipoperoxidation inhibitors containing the above-mentioned heterocyclic derivative or a pharmaceutically acceptable salt thereof.

In the present specification, each symbol denotes the following.

Lower alkyl at R¹, R², R³, R⁴, R⁸, R¹¹, R¹² and R¹³ may be linear or branched and preferably has 1 to 4 carbon atoms. Examples thereof include methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl and the like.

Lower alkoxy at R¹, R², R³, R⁴, R¹¹, R¹² and R¹³ may be linear or branched and preferably has 1 to 4 carbon atoms. Examples thereof include methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, sec-butoxy, tert-butoxy and the like.

Alkyl at R⁵, R⁶, R⁶′, R⁷ and R¹⁰ may be linear or branched and preferably has 1 to 12 carbon atoms. Examples thereof include methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, isopentyl, neopentyl, hexyl, heptyl, octyl, nonyl, decyl, undecyl, dodecyl, 1,1-dimethylpropyl, 1,1-dimethylbutyl, 1,1-dimethylpentyl, 1,1-dimethylhexyl, 3,3-dimethylbutyl, 4,4-dimethylbutyl and the like.

Cycloalkyl at R⁵, R⁶, R⁶′ and R⁷ preferably has 3 to 6 carbon atoms. Examples thereof include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and the like.

With regard to cycloalkylalkyl at R⁵, R⁶, R⁶′ and R⁷, its cycloalkyl moiety preferably has 3 to 6 carbon atoms and alkyl moiety preferably has 1 to 3 carbon atoms. Examples of cycloalkylalkyl include cyclopropylmethyl, cyclobutylmethyl, cyclopentylmethyl, cyclohexylmethyl, cyclopropylethyl, cyclopropylpropyl and the like.

Examples of aryl at R⁵, R⁶, R⁶′ and R⁷ include phenyl, naphthyl and the like.

Arylalkyl at R⁵, R⁶, R⁶′ and R⁷ has an aryl moiety as exemplified above and its alkyl moiety preferably has 1 to 4 carbon atoms. Examples of arylalkyl include benzyl, 1-phenylethyl, 2-phenylethyl, 1-phenylpropyl, 2-phenylpropyl, 3-phenylpropyl and the like.

Heterocycle group at R⁵, R⁶, R⁶′ and R¹⁰ is a monovalent group which occurs as a result of liberation of one hydrogen atom bonded to the ring of heterocyclic compound and may be aliphatic or aromatic. Examples thereof include pyrrolidinyl, piperidyl, piperidino, morpholinyl, morpholino, piperazinyl, pyrrolyl, imidazolyl, pyridyl and the like.

Heterocyclic alkyl at R⁵, R⁶, R⁶′ and R¹⁰ has a heterocyclic moiety as exemplified above and its alkyl moiety preferably has 1 to 8 carbon atoms. Examples thereof include (1-pyrrolidinyl)butyl, morpholinopropyl, 1,1-dimethyl-2-(1-pyrrolidinyl)ethyl, 1,1-dimethyl-2-piperidinoethyl, 1,1-dimethyl-3-(imidazol-1-yl)propyl, (2,6-dimethylpiperidino)methyl, (2,6-dimethylpiperidino)ethyl, (2,6-dimethylpiperidino)propyl and the like.

The above-mentioned alkyl, cycloalkyl, cycloalkylalkyl, aryl, arylalkyl, heterocycle and heterocyclic alkyl may be substituted. Examples of the substituent include alkyl, amino, hydroxy, dialkylamino, aminoalkyl, alkoxy, carboxyl, alkoxycarbonyl, carboxyalkyl, acyloxy, phenyl, phenoxy, halogen atom and the like.

Alkyl in alkyl, dialkylamino, aminoalkyl and carboxyalkyl is exemplified by the above-mentioned lower alkyl. Alkoxy in alkoxy and alkoxycarbonyl is exemplified by the above-mentioned lower alkoxy. Acyloxy may be linear or branched and preferably has 2 to 5 carbon atoms. Examples thereof include acetyloxy, propionyloxy, butyryloxy, valeryloxy, pivaloyloxy and the like. Halogen atom is exemplified by those to be mentioned later. Alkyl in dialkylamino may be substituted by phenyl.

Alkenyl at R⁵ may be linear or branched and preferably has 2 to 8 carbon atoms. Examples thereof include ethenyl, propenyl, isopropenyl, butenyl, pentenyl, hexenyl, heptenyl, octenyl, 3,3-dimethyl-2-propenyl and the like.

Alkynyl at R⁵ may be linear or branched and preferably has 2 to 8 carbon atoms. Examples thereof include ethynyl, propynyl, butynyl, pentynyl, hexynyl, heptynyl, octynyl, 3,3-dimethyl-2-propynyl and the like.

Alkyl moiety of dialkylaminoacyloxyalkyl at R⁵ preferably has 1 to 8 carbon atoms, and its acyl moiety may be linear or branched and preferably has 2 to 5 carbon atoms. Examples thereof include acetyl, propionyl, butyryl, valeryl, pivaloyl and the like. The dialkylaminoacyloxyalkyl is specifically exemplified by N,N-dimethylaminoacetoxyethyl, N,N-dimethylaminoacetoxypropyl and the like.

Alkylene at R^(A), R^(B), R^(D), R^(E) and R⁹ may be linear or branched and preferably has 1 to 8 carbon atoms. Examples thereof include methylene, ethylene, trimethylene, propylene, tetramethylene, pentamethylene, hexamethylene, 1,1-dimethylethylene, 2,2-dimethylpropylene and the like.

Alkali metal at A, B, D and E is preferably sodium, potassium and the like.

Halogen atom at X is exemplified by chlorine atom, bromine atom, iodine atom and the like.

When R⁴ —NHCO—R⁶, R⁶ and R⁵ may combinedly form a ring. The group (—R⁶—R⁵—) formed by R⁶ and R⁵ in combination may be linear or branched and preferably has 2 to 12 carbon atoms. Examples thereof include alkylene such as 1,1-dimethyltrimethylene, 1,1-dimethyltetramethylene, 2,2-dimethyltetramethylene, 1,1-dimethylpentamethylene, 2,2-dimethylpentamethylene and the like, and alkylene havig —OCO— bond, such as —C(CH₃)₂CH₂OCO(CH₂)₃—, —C(CH₃)₂CH₂OCOC(CH₃)₂(CH₂)₃— and the like.

The preferable Compound (I) of the present invention includes, for example,

1-butyl-3-(1-hexyl-4,6-dimethylindolin-5-yl)urea,

1-butyl-3-(1-hexyl-4,6-dimethylindolin-7-yl)urea,

N-(1-hexyl-4,6-dimethylindolin-5-yl)-2,2-dimethylpropanamide,

N-(1-hexyl-4,6-dimethylindolin-7-yl)-2,2-dimethylpropanamide,

N-(1-pentyl-4,6-dimethylindolin-7-yl)-2,2-dimethylpropanamide,

N-(1-isobutyl-4,6-dimethylindolin-7-yl)-2,2-dimethylpropanamide,

N-(1-hexyl-4,6-dimethylindolin-7-yl)-2,2-dimethylbutanamide,

N-(1-hexyl-4,6-dimethylindolin-7-yl)-2,2-dimethylpentanamide,

N-(1-hexyl-4,6-dimethylindolin-7-yl)-cyclohexanamide,

N-(1-hexyl-4,6-dimethylindolin-7-yl)-2,2-dimethyl-3-ethoxypropanamide,

N-(1-ethoxypropyl-4,6-dimethylindolin-7-yl)-2,2-dimethylpropanamide,

N-(1-hexyl-4,6-dimethylindolin-7-yl)-2,2-dimethyl-3-piperidinopropanamide,

N-(1-piperidinopropyl-4,6-dimethylindolin-7-yl)-2,2-dimethylpropanamide,

N-(1-hexyl-4,6-dimethylindolin-7-yl)-2,6-dimethylpiperidinopropanamide, and the like, and pharmaceutically acceptable salts thereof.

The Compound (I) may be converted to a pharmaceutically acceptable salt thereof.

The Compound (I) may be converted to an acid addition salt, since it has a basic group, and the acid to form this acid addition salt includes, for example, an inorganic acid such as hydrochloric acid, sulfuric acid, phosphoric acid, nitric acid and the like; an organic acid such as oxalic acid, fumaric acid, maleic acid, citric acid, tartaric acid, methanesulfonic acid, toluenesulfonic acid and the like; and the like.

When Compound (I) has an acidic group such as carboxyl, it can form an alkali metal salt such as sodium salt, potassium salt and the like; alkaline earth metal salt such as calcium salt, magnesium salt and the like; organic base salt such as triethylamine salt, dicyclohexylamine salt, pyridine salt and the like; and the like.

The Compound (I) and pharmaceutically acceptable salts thereof can be produced, for example, by the following methods.

Production Method 1

Compound (II) and compound (III) are reacted.

This method produces a compound of the formula (I) wherein R⁶ is —NR⁷R⁸ where R⁸ is hydrogen atom.

This reaction generally proceeds in an inert solvent. Examples of the inert solvent include acetone, dioxane, acetonitrile, chloroform, benzene, methylene chloride, ethylene chloride, tetrahydrofuran, ethyl acetate, N,N-dimethylformamide, pyridine, water and the like, and mixtures thereof.

In addition, a base such as triethylamine, pyridine, 4-dimethylaminopyridine, potassium carbonate and the like may be added.

The reaction temperature is generally from −10° C. to 160° C., preferably 20-100° C., and the reaction time is generally from 30 minutes to 10 hours.

The starting compound (II) can be prepared, for example, by the following method.

A nitro group is introduced into a compound of the general formula (X)

wherein R¹¹, R¹², R¹³ and m are as defined above and R¹⁴ is an amino-protecting group [see J. Eric. Mordlander, et al., J. Org. Chem., 46, 778-782 (1981)], (introduction of nitro onto benzene ring) using nitric acid in a mixed solvent of acetic acid and sulfuric acid, and the amino-protecting group is eliminated. The compound thus obtained and compound (IX) are reacted, and nitro group is reduced using a catalyst such as palladium-carbon and the like to give starting compound (II).

Examples of the amino-protecting group at R¹⁴ include acyl such as formyl, acetyl, monochloroacetyl, dichloroacetyl, trichloroacetyl, trifluoroacetyl, propionyl, benzoyl and the like.

Said amino-protecting group is eliminated by a method known per se. For example, it is eliminated by the action of an acid (e.g., hydrochloric acid, formic acid, trifluoroacetic acid and the like) or an alkali (e.g., sodium hydroxide, potassium hydroxide, sodium carbonate, sodium hydrogencarbonate and the like), or other method.

Production Method 2

Compound (II) and compound (IV) are reacted.

This method produces a compound of the formula (I) wherein R⁶ can be any one of those defined above.

This reaction generally proceeds in an inert solvent. Examples of the inert solvent include acetone, dioxane, acetonitrile, chloroform, benzene, methylene chloride, ethylene chloride, tetrahydrofuran, ethyl acetate, N,N-dimethylformamide, pyridine, water and the like, and mixtures thereof.

In addition, a base such as triethylamine, pyridine, 4-dimethylaminopyridine, potassium carbonate and the like may be added.

The reaction temperature is generally from −10° C. to 100° C., preferably 0-60° C., and the reaction time is generally from 30 minutes to 10 hours.

Production Method 3

Compound (II) and compound (V) or a reactive derivative thereof are reacted.

This method produces a compound of the formula (I) wherein R⁶ is R⁶′.

This reaction generally proceeds in an inert solvent. Examples of the inert solvent include acetone, dioxane, acetonitrile, chloroform, benzene, methylene chloride, ethylene chloride, tetrahydrofuran, ethyl acetate, N,N-dimethylformamide, pyridine, water and the like, and mixtures thereof.

In addition, a base such as triethylamine, pyridine, 4-dimethylaminopyridine, potassium carbonate and the like may be added.

The reaction temperature is generally from −10° C. to 100° C., preferably 0-60° C., and the reaction time is generally from 30 minutes to 10 hours.

Compound (V) is subjected to said reaction as, for example, a free acid; a salt such as sodium, potassium, calcium, triethylamine, pyridine and the like; or a reactive derivative such as acid anhydride, mixed acid anhydride [e.g., substituted phosphoric acid (e.g., dialkylphosphoric acid), alkyl carbonate (e.g., monoethyl carbonate) and the like], active amide (e.g., amide with imidazole etc.), ester (e.g., cyanomethyl ester, 4-nitrophenyl ester and the like), and the like.

When Compound (V) is used as a free acid or salt in this reaction, the reaction is preferably carried out in the presence of a condensing agent. Examples of the condensing agent include dehydrating agent such as N,N′-di-substituted-carbodiimides (e.g., N,N′-dicyclohexylcarbodiimide), carbodiimide compounds (e.g., 1-ethyl-3-(3′-dimethylaminopropyl)carbodiimide, N-cyclohexyl-N′-morpholinoethylcarbodiimide and N-cyclohexyl-N′-(4-diethylaminocyclohexyl)carbodiimide), azolide compounds (e.g., N,N′-carbonyldiimidazole and N,N′-thionyldiimidazole) and the like. When these condensing agents are used, the reaction is considered to proceed via a reactive derivative of carboxylic acid.

Production Method 4

Compound (VI) and compound (VII) are reacted.

This method produces a compound of the formula (I) wherein R⁶ is —NR⁷R⁸.

This reaction generally proceeds in an inert solvent. Examples of the inert solvent include acetone, dioxane, acetonitrile, chloroform, benzene, methylene chloride, ethylene chloride, tetrahydrofuran, ethyl acetate, N,N-dimethylformamide, pyridine, water and the like, and mixtures thereof.

In addition, a base such as triethylamine, pyridine, 4-dimethylaminopyridine, potassium carbonate and the like may be added.

The reaction temperature is generally from −10° C. to 160° C., preferably 10-100° C., and the reaction time is generally from 30 minutes to 10 hours.

The starting compound (VI) can be produced, for example, by dissolving compound (II) in an inert solvent and bubbling in phosgene.

Production Method 5

Compound (VIII) and compound (IX) are reacted.

This reaction generally proceeds in an inert solvent. Examples of the inert solvent include acetone, dioxane, acetonitrile, chloroform, benzene, methylene chloride, ethylene chloride, tetrahydrofuran, ethyl acetate, N,N-dimethylformamide, pyridine, water and the like, and mixtures thereof.

In addition, a base such as triethylamine, pyridine, 4-dimethylaminopyridine, potassium carbonate, sodium hydride and the like may be added.

The reaction temperature is generally from −10° C. to 100° C., preferably 0-60° C., and the reaction time is generally from 30 minutes to 10 hours.

The starting compound (VIII) can be prepared, for example, by the method wherein a nitro group is introduced into a compound of the formula (X) (introduction of nitro onto benzene ring), and the nitro group is reduced using a catalyst such as palladium-carbon and the like to give a compound of the formula (XI)

wherein R¹¹, R¹², R¹³, R¹⁴ and m are as defined above. Using this compound as a starting compound and according to Production Method 2, a compound of the formula (XII)

wherein R¹, R^(2,) R³, R⁴, R¹⁴ and m are as defined above, is obtained. This compound is deprotected to give compound (VIII).

The Compound (I) of the present invention obtained as in the above can be purified by a method conventionally known, such as chromatography and recrystallization.

This Compound (I) can be converted to a pharmaceutically acceptable salt by a method known per se.

The Compound (I) and pharmaceutically acceptable salts thereof of the present invention show superior ACAT inhibitory activity and lipoperoxidation inhibitory activity in mammals (e.g., human, cow, horse, dog, cat, rabbit, rat, mouse, hamster and the like) and are useful as ACAT inhibitors and hyperlipemia inhibitors. To be specific, they are useful for the prevention and treatment of arteriosclerotic lesions such as arteriosclerosis, hyperlipemia and diabetes, as well as ischemic diseases of brain, heart and the like.

A pharmaceutical composition containing Compound (I) or a pharmaceutically acceptable salt thereof of the present invention may contain an additive. Examples of the additive include excipients (e.g., starch, lactose, sugar, calcium carbonate and calcium phosphate), binders (e.g., starch, gum arabic, carboxymethylcellulose, hydroxypropylcellulose and crystalline cellulose), lubricants (e.g., magnesium stearate and talc), disintegrators (e.g., calcium carboxymethylcellulose and talc), and the like.

The above-mentioned ingredients are mixed, and the mixture can be formulated into an oral preparation such as capsule, tablet, fine granules, granules, dry syrup and the like, or a parenteral preparation such as injection, suppository and the like by a method conventionally known.

While the dose of Compound (I) and pharmaceutically acceptable salts thereof of the present invention varies depending on administration target, symptom and other factors, it is generally about 0.1-50 mg/kg body weight per dose for an adult patient with hypercholesterolemia by oral administration in about one to three times a day.

The present invention is described in more detail in the following by way of Examples, to which the present invention is not limited.

EXAMPLE 1

1-butyl-3-(1-hexyl-4,6-dimethylindolin-5-yl)urea

(1) 1-acetyl-4,6-dimethylindoline 4,6-Dimethylindole (1.08 g) was dissolved in acetic acid (20 ml), and sodium cyanoborohydride (2.3 g) was added portionwise at 15° C. The mixture was stirred at said temperature for one hour and poured into ice water. Saturated aqueous sodium hydrogencarbonate was added to neutralize the mixture and the mixture was extracted with ethyl acetate. The extract was washed with saturated brine and dried over sodium sulfate. The solvent was evaporated under reduced pressure. The residue was dissolved in benzene, and acetic anhydride (840 mg) was added, which was followed by stirring at room temperature for one hour. The reaction mixture was washed with saturated aqueous sodium hydrogencarbonate and saturated brine, and dried over sodium sulfate. The solvent was evaporated under reduced pressure. The residue was purified by silica gel column chromatography (developing solvent:chloroform-methanol=1:0-10:1) to give 1.3 g of the title compound (1).

¹H—NMR (CDCl₃) δ: 2.18 (6H, s, —CH₃, —COCH₃), 2.30 (3H, s, —CH₃), 3.00 (2H, t, J=8.3 Hz, C₃—H₂), 4.03 (2H, t, J=8.3 Hz, C₂—H₂), 6.66 (1H, s, C₅—H), 7.89 (1H, s, C₇—H)

(2) 1-acetyl-4,6-dimethyl-5-nitroindoline

1-Acetyl-4,6-dimethylindoline (2.6 g) was dissolved in acetic anhydride (35 ml), and nitric acid (d=1.5, 0.92 ml) dissolved in acetic anhydride (15 ml) was added dropwise at 0° C. The mixture was stirred at room temperature for one hour and poured into ice water. Saturated aqueous sodium hydrogencarbonate was added to neutralize the mixture, and the mixture was extracted with chloroform. The extract was washed with saturated brine and dried over sodium sulfate. The solvent was evaporated under reduced pressure. The residue was purified by silica gel column chromatography (developing solvent:chloroform-methanol=1:0-100:1) to give 2.4 g of the title compound (2).

¹H—NMR (CDCl₃) δ: 2.17 (3H, s, —COCH₃), 2.24 (3H, s, —CH₃), 2.30 (3H, s, —CH3), 3.08 (2H, t, J=8.4 Hz, C₃—H₂), 4.14 (2H, t, J=8.3 Hz, C₂—H₂), 8.00 (1H, s, C₇—H)

(3) 4,6-dimethyl-1-hexyl-5-nitroindoline

1-Acetyl-4,6-dimethyl-5-nitroindoline (2.4 g) obtained in (2) was dissolved in methanol. (25 ml) and 6N hydrochloric acid (20 ml) was added, which was followed by refluxing for 15 hours. After the completion of the reaction, the solvent was evaporated under reduced pressure. The residue was dissolved in chloroform, and the mixture was washed with saturated aqueous sodium hydrogencarbonate and saturated brine, and dried over sodium sulfate. The solvent was evaporated under reduced pressure. Indoline (1.8 g) thus obtained was dissolved in dimethylformamide (20 ml), and sodium hydride (abt. 60% in oil suspension, 457 mg) was added at 0° C. The mixture was stirred at said temperature for 0.5 hour and hexyl bromide (1.8 g) was added to the reaction mixture, which was followed by stirring at room temperature for 3 hours. Water was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The extract was washed with saturated brine and dried over sodium sulfate. The solvent was evaporated under reduced pressure. The residue was purified by silica gel column chromatography (developing solvent:hexane-ethyl acetate=1:0-10:1) to give 2.8 g of the title compound (3).

¹H—NMR (CDCl₃) δ: 0.90 (3H, br-t, —CH₃), 1.2-1.8 (8H, m, —(CH₂)₄—), 2.17 (3H, s, —CH₃), 2.30 (3H, s, —CH₃), 3.00 (2H, t, J=8.4 Hz, C₃—H₂), 3.09 (2H, t, J=7.2 Hz, N—CH₂), 3.51 (2H, t, J=8.3 Hz, C₂—H₂), 5.99 (1H, s, C₇—H)

(4) 1-butyl-3-(1-hexyl-4,6-dimethylindolin-5-yl)urea

4,6-Dimethyl-1-hexyl-5-nitroindoline (1.0 g) obtained in (3) was dissolved in benzene (40 ml) and 10% palladium-carbon (100 mg) was added to allow hydrogenation at 40° C. After the completion of the reaction, palladium-carbon was filtered off, and the filtrate was washed with saturated aqueous sodium hydrogencarbonate and saturated brine, and dried over sodium sulfate. The solvent was evaporated under reduced pressure. 5-Amino-4,6-dimethyl-1-hexylindoline thus obtained was dissolved in chloroform (20 ml) and butyl isocyanate (400 mg) was added to the reaction mixture, which was followed by stirring at room temperature for 18 hours. Water was added to the reaction mixture, and the mixture was washed with saturated brine and dried over sodium sulfate. The solvent was evaporated under reduced pressure. The residue was purified by silica gel column chromatography (developing solvent:chloroform-methanol=1:0-50:1) and recrystallized from ethanol to give 650 mg of the title compound (4).

¹H—NMR (CDCl₃) δ: 0.91 (6H, br-t, —CH₃), 1.0-2.0 (12H, m, —(CH₂)₄—, —(CH₂)₂—), 2.09 (3H, s, —CH₃), 2.19 (3H, s, —CH₃), 2.6-3.6 (8H, m, NH—CH₂, C₃—H₂, N—CH₂, C₂—H₂), 4.29 (1H, br, NH), 5.45 (1H, br, NH), 6.18 (1H, s, C₇—H)

EXAMPLE 2

N-(1-hexyl-4,6-dimethylindolin-5-yl)-2,2-dimethylpropanamide

5-Amino-4,6-dimethyl-1-hexylindoline (880 mg) was dissolved in chloroform (20 ml), and triethylamine (370 mg) and pivaloyl chloride (430 mg) were added, which was followed by stirring at room temperature for 1 hour. Water was added to the reaction mixture, and the mixture was washed with saturated brine and dried over sodium sulfate. The solvent was evaporated under reduced pressure. The residue was purified by silica gel column chromatography (developing solvent:chloroform-methanol=1:0-50:1) and recrystallized from ethanol to give 650 mg of the title compound.

¹H—NMR (CDCl₃) δ: 0.84 (3H, br-t, —CH₃), 1.1-1.8 (8H, m, —(CH₂)₄—), 1.33 (9H, s, —(CH₃)₃), 2.00 (3H, s, —CH₃), 2.11 (3H, s, —CH₃), 2.82 (2H, t, J=7.8 Hz, C₃—H₂), 2.99 (2H, t, J=7.2 Hz, N—CH₂), 3.33 (2H, t, J=7.8 Hz, C₂—H₂), 6.16 (1H, s, C₇—H), 6.70 (1H, br, N—H)

EXAMPLE 3

N-(1-hexyl-4,6-dimethylindolin-7-yl)-2,2-dimethylpropanamide

(1) 1-acetyl-5-bromo-4,6-dimethylindoline

1-Acetyl-4,6-dimethylindoline (5.5 g) was dissolved in acetic acid (150 ml), and bromine (2.2 ml) was added dropwise at room temperature. The mixture was stirred at room temperature for 1 hour, and poured into ice water. The precipitated solid was collected by filtration, and recrystallized from methanol to give 6.5 g of the title compound (1).

¹H—NMR (CDCl₃) δ: 2.19 (3H, s, —COCH₃), 2.27 (3H, s, —CH₃), 2.39 (3H, s, —CH₃), 3.06,(2H, t, J=8.4 Hz, C₃—H₂), 4.03 (2H, t, J=8.4 Hz, C₂—H₂), 7.99 (1H, s, C₇—H)

(2) 5-bromo-4,6-dimethyl-7-nitroindoline

Concentrated sulfuric acid (25 ml) and nitric acid (d=1.56, 1.46 ml) were added to acetic acid (25 ml), and 1-acetyl-5-bromo-4,6-dimethylindoline (6.5 g) obtained in (1) was added while stirring the mixture at 0° C., which was followed by stirring at said temperature for 18 hours. The reaction mixture was poured into ice water, and the precipitated solid was collected by filtration and washed thoroughly with water. The obtained solid was suspended in ethanol (50 ml) and water (10 ml). Sodium hydroxide (20 g) was added and the mixture was refluxed for 3 hours. The solvent was evaporated under reduced pressure and chloroform was added. The mixture was washed with saturated brine and dried over sodium sulfate. The solvent was evaporated under reduced pressure. The residue was purified by silica gel column chromatography (developing solvent:benzene) to give 4.8 g of the title compound (2).

¹H—NMR (CDCl₃) δ: 2.29 (3H, s, C₄—CH₃), 2.65 (3H, s, C₆—CH₃), 3.10 (2H, t, J=8.4 Hz, C₃—H₂), 3.82 (2H, t, J=8.4 Hz, C₂—H₂), 9.0 (1H, br, N—H)

(3) N-(1-hexyl-4,6-dimethylindolin-7-yl)-2,2-dimethylpropanamide

5-Bromo-4,6-dimethyl-7-nitroindoline (3.0 g) obtained in (2) was dissolved in dimethylformamide (60 ml) and sodium hydride (abt. 60% in oil suspension, 530 mg) was added at 0° C., which was followed by stirring at said temperature for 0.5 hour. Hexyl bromide (1.8 g) was added to the reaction mixture and the mixture was stirred at room temperature for 12 hours. Water was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The extract was washed with saturated brine and dried over sodium sulfate. The solvent was evaporated under reduced pressure. The residue was purified by silica gel column chromatography (developing solvent:hexane-ethyl acetate=1:0-10:1). The obtained solid was dissolved in benzene (40 ml) and 10% palladium-carbon (100 mg) was added to allow hydrogenation at 40° C. After the completion of the reaction, palladium-carbon was filtered off, and the filtrate was washed with saturated aqueous sodium hydrogencarbonate and saturated brine, and dried over sodium sulfate. The solvent was evaporated under reduced pressure.

7-Amino-4,6-dimethyl-1-hexylindoline thus obtained was dissolved in chloroform (20 ml), and triethylamine (1.0 g) and pivaloyl chloride (1.0 g) were added to the reaction mixture, which was followed by stirring at room temperature for 1 hour. Water was added to the reaction mixture, and the mixture was washed with saturated brine and dried over sodium sulfate. The solvent was evaporated under reduced pressure. The residue was purified by silica gel column chromatography (developing solvent:chloroform-methanol=1:0-50:1). The obtained compound (3) was dissolved in ethanol and 10N hydrochloric acid/ethanol (1 ml) was added. The solvent was evaporated under reduced pressure. The residue was recrystallized from ethanol to give 700 mg of hydrochloride of the title compound (3).

¹H—NMR (CDCl₃) δ: 0.90 (3H, br-t, —CH₃), 1.1-1.6 (8H, m, —(CH₂)₄—), 1.41 (9H, s, —(CH₃)₃), 2.15 (3H, s, —CH₃), 2.25 (3H, s, —CH₃), 3.15 (4H, m, C₃—H₂, N—CH₂), 3.70 (1H, m, C₂—H), 4.00 (1H, m, C₂—H), 7.12 (1H, s, C₅—H), 9.2 (1H, br, N—H)

EXAMPLE 4

1-butyl-3-(1-hexyl-4,6-dimethylindolin-7-yl)urea

7-Amino-4,6-dimethyl-1-hexylindoline (800 mg) was dissolved in chloroform (20 ml) and butyl isocyanate (400 mg) was added, which was followed by stirring at room temperature for 18 hours. Water was added to the reaction mixture, and the mixture was washed with saturated brine and dried over sodium sulfate. The solvent was evaporated under reduced pressure. The residue was purified by silica gel column chromatography (developing solvent:chloroform-methanol=1:0-50:1) and recrystallized from ethanol to give 450 mg of the title compound.

¹H—NMR (CDCl₃) δ: 0.88 (6H, br-t, —CH₃), 1.0-1.8 (12H, m, —(CH₂)₄—, —(CH₂)₂—), 2.13 (6H, s, —CH₃×2), 2.83 (2H, t, J=8.3 Hz, C₃—H₂), 3.20 (4H, N—CH₂×2), 3.43 (2H, t, J=8.3 Hz, C₂—H₂), 4.80 (1H, br-t, NH—CH₂), 5.52 (1H, br-s, C₇—NH), 6.40 (1H, s, C₅—H)

EXAMPLES 5-36

In the same manner as in any one of the above-mentioned Examples 1-4, the compounds shown in Tables 1 and 2 were obtained.

TABLE 1

Example R¹ R² R³ R⁶ R⁵  5 —H —H —CH₃ —C(CH₃)₃ —(CH₂)₅CH₃   6 —CH₃ —H —CH₃ —C(CH₃)₂(CH₂)₃CH₃ —CH₂CH₃   7 —OCH₃ —H —OCH₃ —C(CH₃)₃ —(CH₂)₅CH₃   8 —CH₃ —H —CH₃ —C(CH₃)₃ —(CH₂)₃CH₃   9 —CH₃ —H —CH₃ —C(CH₃)₂(CH₂)₃CH₃ —(CH₂)₃CH₃ 10 —CH₃ —H —CH₃ —C(CH₃)₂(CH₂)₅CH₃ —(CH₂)₃CH₃ 11 —CH₃ —H —CH₃ —C(CH₃)₃ —(CH₂)₇CH₃ 12 —CH₃ —H —CH₃ —C(CH₃)₃ —CH₂CH₃ 13 —CH₃ —H —CH₃ —C(CH₃)₂(CH₂)₅CH₃ —CH₂CH₃ 14 —CH₃ —H —CH₃ —C(CH₃)₂(CH₂)₃CH₃ —(CH₂)₅CH₃ 15 —CH₃ —H —CH₃ —C(CH₃)₂(CH₂)₅CH₃ —(CH₂)₅CH₃ 16 —CH₃ —H —CH₃ —CH₃ —(CH₂)₅CH₃ 17 —CH₃ —H —CH₃ —CH(CH₃)₂ —(CH₂)₅CH₃ 18 —CH₃ —H —CH₃ —CH₂C(CH₃)₂CH₂COOH —(CH₂)₅CH₃ 19 —CH₂CH₃ —H —CH₂CH₃ —C(CH₃)₃ —(CH₂)₅CH₃ 20 —CH₃ —H —CH₃ —C(CH₃)₂(CH₂)₂COOH —(CH₂)₅CH₃

TABLE 2

Example R¹ R² R³ R⁶ R⁵ 21 —CH₃ —H —CH₃ —(CH₂)₃CH₃ —(CH₂)₅CH₃ 22 —CH₃ —H —CH₃ —C(CH₃)₃ —(CH₂)₂OCOCH₃ 23 —CH₃ —H —CH₃ —C(CH₃)₃ —(CH₂)₂OCH₃ 24 —CH₃ —H —CH₃ —C(CH₃)₃ —(CH₂)₂OC₂H₅ 25 —CH₃ —H —CH₃ —C(CH₃)₃ —C₅H₁₁ 26 —CH₃ —H —CH₃ —C(CH₃)₃ —(CH₂)₅COOH 27 —CH₃ —H —CH₃ —C(CH₃)₃ —C₇H₁₅ 28 —CH₃ —H —CH₃ —C(CH₃)₂CH₂OH —C₆H₁₃ 29 —CH₃ —H —CH₃ —C(CH₃)₂CH₂OCOCH₃ —C₆H₁₃ 30 —CH₃ —H —CH₃

—C₆H₁₃ 31 —CH₃ —H —CH₃ —C(CH₃)₃ —(CH₂)₂CH(CH₃)₂ 32 —CH₃ —H —CH₃ —C(CH₃)₃ —CH₂CH(CH₃)₂ 33 —CH₃ —H —CH₃

—C₆H₁₃ 34 —CH₃ —H —CH₃ —C(CH₃)₂CH₂OC₂H₅ —C₆H₁₃ 35 —CH₃ —H —CH₃

—C₆H₁₃ 36 —CH₃ —H —CH₃ —C(CH₃)₃ —C₃H₇

The ¹H—NMR data of the compounds of the above Examples 5-36 are shown in the following.

¹H—NMR (CDCl₃) δ:

EXAMPLE 5

(Hydrochloride)

0.91 (3H, br-t), 1.1-1.8 (8H, m), 1.34 (9H, s), 2.09 (3H, s), 2.93-3.48 (4H, m), 3.48-3.82 (1H, m), 3.82-4.35 (1H, m), 7.12 (1H, d), 7.32 (1H, d), 9.36 (1H, br-s)

EXAMPLE 6

0.93 (3H, br-t), 1.0-2.0 (19H, m), 1.35 (6H, s), 2.12 (3H, s), 2.19 (3H, s), 2.98 (2H, t), 3.25 (2H, t), 3.65 (2H, t), 6.80 (1H, s), 8.09 (1H, br-s)

EXAMPLE 7

0.87 (3H, br-t), 1.1-1.8 (8H, m), 1.31 (9H, s), 2.84 (2H, t), 3.16 (2H, t), 3.44 (2H, t), 3.74 (3H, s), 3.79 (3H, s), 5.86 (1H, s)

EXAMPLE 8

0.92 (3H, br-t), 1.39 (9H, s), 1.2-1.9 (4H, m), 2.08 (3H, s), 2.15 (3H, s), 2.80-3.30 (4H, m), 3.60 (2H, t), 6.68 (1H, s), 7.78 (1H, br-s)

EXAMPLE 9

0.90 (3H, br-t), 0.92 (3H, br-t), 1.08-1.88 (10H, m), 1.29 (6H, s), 2.05 (3H, s), 2.12 (3H, s), 2.81 (2H, t), 3.13 (2H, t), 3.40 (2H, t), 6.39 (1H, s), 6.74 (1H, br-s)

EXAMPLE 10

0.63-1.05 (6H, m), 1.05-1.82 (14H, m), 1.29 (6H, s), 2.08 (3H, s), 2.10 (3H, s), 2.82 (2H, s), 3.13 (2H, t), 3.41 (2H, t), 6.41 (1H, s), 6.77 (1H, br-s)

EXAMPLE 11

0.88 (3H, br-t), 1.01-1.87 (12H, m), 1.35 (9H, s), 2.09 (3H, s), 2.14 (3H, s), 2.82 (2H, t), 3.13 (2H, t), 3.52 (2H, t), 6.60 (1H, br-s)

EXAMPLE 12

1.18 (3H, t), 1.35 (9H, s), 2.09 (3H, s), 2.15 (3H, s), 2.92 (2H, t), 3.22 (2H, q), 3.56 (2H, t), 6.62 (1H, s), 7.57 (1H, br-s)

EXAMPLE 13

0.88 (3H, br-t), 1.08 (3H, t), 1.29 (6H, s), 1.1-1.9 (10H, m), 2.08 (3H, s), 2.22 (3H, s), 2.82 (2H, t), 3.21 (2H, q), 3.33 (2H, t), 6.43 (1H, s), 6.84 (1H, br-s)

EXAMPLE 14

0.90 (3H, br-t), 0.92 (3H, br-t), 1.08-1.88 (14H, m), 1.29 (6H, s), 2.05 (3H, s), 2.12 (3H, s), 2.81 (2H, t), 3.13 (2H, t), 3.40 (2H, t), 6.39 (1H, s), 6.74 (1H, br-s)

EXAMPLE 15

0.63-1.05 (6H, m), 1.05-1.82 (18H, m), 1.34 (6H, s), 2.11 (3H, s), 2.17 (3H, s), 2.95 (2H, s), 3.13 (2H, t), 3.60 (2H, t), 6.72 (1H, s), 7.8 (1H, br)

EXAMPLE 16

0.89 (3H, br-t), 1.05-1.70 (8H, m), 1.81 (3H, s), 2.11 (3H, s), 2.15 (3H, s), 2.81 (2H, br-t), 2.6-3.8 (4H, m), 6.39 (1H, s), 6.58 (1H, br), 6.72 (1H, br)

EXAMPLE 17

0.87 (3H, br-t), 1.05-1.90 (8H, m), 1.25 (3H, s), 1.32 (3H, s), 2.11 (3H, s), 2.17 (3H, s), 2.67 (1H, m), 2.7-3.4 (4H, m), 3.59 (2H, t), 6.69 (1H, s), 7.83 (1H, br-s)

EXAMPLE 18

0.87 (3H, br-t), 1.05-1.80 (8H, m), 1.21 (6H, s), 2.17 (6H, s), 2.48 (2H, s), 2.55 (2H, s), 3.01 (2H, t), 3.10 (2H, t), 3.54 (2H, t), 6.70 (1H, s), 6.8-8.2 (1H, br-s), 8.72 (1H, br-s)

EXAMPLE 19

0.87 (3H, br-t), 1.05-1.80 (8H, m), 1.17 (3H, t), 1.20 (3H, t), 1.35 (9H, s), 2.22-2.62 (4H, m), 2.91 (2H, t), 3.04 (2H, t), 3.48 (2H, br-t), 6.56 (1H, s), 7.25 (1H, br-s)

EXAMPLE 20

0.85 (3H, br-t), 1.05-1.80 (8H, m), 1.27 (6H, s), 1.80-2.25 (2H, m), 2.10 (3H, s), 2.16 (3H, s), 2.25-2.55 (2H, m), 2.90 (2H, t), 3.04 (2H, t), 3.47 (2H, t), 6.69 (1H, s), 8.49 (1H, br-s), 8.95 (1H, br-s)

EXAMPLE 21

0.83 (3H, br-t), 0.87 (3H, br-t), 1.1-1.8 (11H, m), 2.11 (3H, s), 2.16 (3H, s), 2.29 (1H, t), 2.85 (2H, t), 3.22 (2H, t), 3.55 (2H, t), 6.49 (1H, s), 6.68 (1H, br)

EXAMPLE 22

1.27 (9H, s), 2.04 (6H, s), 2.13 (3H, s), 3.06 (2H, t), 3.43 (2H, t), 3.75 (2H, t), 4.29 (2H, t), 6.87 (1H, s), 9.15 (1H, br-s)

EXAMPLE 23

1.33 (9H, s), 2.04 (3H, s), 2.09 (3H, s), 2.8-2.9 (4H, m), 3.38 (3H, s), 3.4-3.6 (4H, m), 6.36 (1H, s), 7.35 (1H, br-s)

EXAMPLE 24

1.16 (3H, t), 1.32 (9H, s), 2.04 (3H, s), 2.09 (3H, s), 2.82 (2H, t), 3.3-3.6 (8H, m), 6.53 (1H, s), 7.40 (1H, br-s)

EXAMPLE 25

0.88 (3H, br-t), 1.35 (9H, s), 1.2-1.9 (6H, m), 2.08 (3H, s), 2.15 (3H, s), 2.92 (2H, t), 3.13 (2H, t), 3.55 (2H, t), 6.23 (1H, s), 7.60 (1H, br-s)

EXAMPLE 26

1.1-1.9 (6H, m), 1.39 (9H, s), 2.07 (3H, s), 2.13 (3H, s), 2.29 (2H, t), 2.84 (2H, t), 3.11 (2H, t), 3.43 (2H, t), 5.40 (1H, br), 6.52 (1H, s), 7.30 (1H, br-s)

EXAMPLE 27

0.87 (3H, br-t), 1.1-1.8 (10H, m), 1.34 (9H, s), 2.07 (3H, s), 2.13 (3H, s), 2.87 (2H, t), 3.13 (2H, t), 3.49 (2H, t), 6.52 (1H, s), 7.20 (1H, br-s)

EXAMPLE 28

0.87 (3H, br-t), 1.1-1.8 (8H, m), 1.26 (6H, s), 1.99 (3H, s), 2.08 (3H, s), 2.78 (2H, t), 3.10 (2H, t), 3.37 (2H, t), 3.48 (2H, s), 4.00 (1H, br-s), 6.35 (1H, s), 7.81 (1H, s)

EXAMPLE 29

0.87 (3H, br-t), 1.1-1.8 (8H, m), 1.28 (6H, s), 2.07 (6H, s), 2.12 (3H, s), 2.84 (2H, t), 3.14 (2H, t), 3.43 (2H, t), 4.20 (2H, s), 6.48,(1H, s), 7.35 (1H, s)

EXAMPLE 30

0.87 (3H, br-t), 1.1-1.8 (8H, m), 2.15 (6H, s), 2.82 (2H, t), 3.16 (2H, t), 3.43 (2H, t), 6.43 (1H, s), 7.42 (2H, m), 8.22 (1H, m), 8.75 (1H, m), 9.16 (1H, br-s)

EXAMPLE 31

0.90 (6H, d), 1.3-1.8 (3H, m), 1.36 (9H, s), 2.09 (3H, s), 2.16 (3H, s), 2.92 (2H, t), 3.16 (2H, t), 3.54 (2H, t), 6.65 (1H, s), 7.65 (1H, br-s)

EXAMPLE 32

0.94 (6H, d), 1.33 (9H, s), 1.7-2.0 (1H, m), 2.04 (3H, s), 2.10 (3H, s), 2.82 (2H, t), 2.94 (2H, t), 3.38 (2H, t), 6.37 (1H, s), 6.70 (1H, br-s)

EXAMPLE 33

0.85 (3H, br-t), 1.1-1.8(8H, m), 1.44 (6H, s), 2.03 (3H, s), 2.08 (3H, s), 2.80 (2H, t), 3.14 (2H, t), 3.41 (2H, t), 4.50 (2H, s), 6.37 (1H, s), 7.04 (1H, br-s), 7.40 (2H, m), 8.28 (1H, m), 8.75 (1H, m), 9.21 (1H, d)

Example 34

0.88 (3H, br-t), 1.1-1.8 (8H, m), 1.23 (3H, t), 1.27 (6H, s), 2.07 (3H, s), 2.10 (3H, s), 2.81 (2H, t), 3.18 (2H, t), 3.41 (2H, t), 3.48 (2H, s), 3.55 (2H, q), 6.37 (1H, s), 8.00 (1H, br-s)

EXAMPLE 35

0.88 (3H, br-t), 1.1-1.8 (14H, m), 1.24 (6H, s), 2.10 (6H, s), 2.50 (2H, t), 2.90 (2H, t), 2.8-3.6 (8H, m), 6.39 (1H, s), 7.00 (1H, br-s)

EXAMPLE 36

0.87 (3H, br-t), 1.2-1.9 (2H, m), 1.35 (9H, s), 2.08 (3H, s), 2.15 (3H, s), 2.93 (2H, t), 3.12 (2H, t), 3.50 (2H, t), 6.30 (1H, s), 7.40 (1H, br-s)

In the same manner as in any one of the above-mentioned Examples 1-4, the compounds shown in Tables 3 to 9 can be obtained.

TABLE 3

Example R¹ R² R³ R⁶ R⁵ 37 —CH₃ —H —CH₃ —C(CH₃)₃ —CH₂—CH═C(CH₃)₂ 38 —CH₃ —H —CH₃ —C(CH₃)₃ —(CH₂)₄SO₃Na 39 —CH₃ —H —CH₃ —C(CH₃)₃

40 —CH₃ —H —CH₃ —C(CH₃)₃

41 —CH₃ —H —CH₃ —C(CH₃)₃

42 —CH₃ —H —CH₃

—CH₂CH₃ 43 —CH₃ —H —CH₃

—(CH₂)₃CH₃ 44 —CH₃ —H —CH₃

—(CH₂)₅CH₃ 45 —CH₃ —H —CH₃

—(CH₂)₅COOH 46 —CH₃ —H —CH₃

—(CH₂)₅CH₃ 47 —CH₃ —H —CH₃

—(CH₂)₅CH₃ 48 —CH₃ —H —CH₃

—(CH₂)₅CH₃ 49 —CH₃ —H —CH₃

—(CH₂)₅CH₃ 50 —CH₃ —H —CH₃

—(CH₂)₅CH₃ 51 —CH₃ —H —CH₃

—CH₂CH₃ 52 —CH₃ —H —CH₃ —(CH₂)₄OC(CH₃)₃ —(CH₂)₃CH₃ 53 —CH₃ —H —CH₃

—(CH₂)₃CH₃

TABLE 4

Example R¹ R² R³ R⁶ R⁵ 54 —CH₃ —H —CH₃

—CH₂CH₃ 55 —CH₃ —H —CH₃

—CH₂CH₃ 56 —CH₃ —H —CH₃

—CH₂CH₃ 57 —CH₃ —H —CH₃ —CH₂C(CH₃)₃ —(CH₂)₅CH₃ 58 —CH₃ —H —CH₃ —C(CH₃)₂(CH₂)₂SO₃Na —(CH₂)₅CH₃ 59 —CH₃ —H —CH₃ —CH₂C(CH₃)₂(CH₂)₂SO₃Na —(CH₂)₅CH₃ 60 —CH₃ —H —CH₃

—(CH₂)₅CH₃ 61 —CH₃ —H —CH₃

—(CH₂)₅CH₃ 62 —H —H —OCH₃ —C(CH₃)₃ —(CH₂)₆CH₃ 63 —H —H —OCH₃ —C(CH₃)₃ —(CH₂)₄CH₃ 64 —H —H —OCH(CH₃)₂ —C(CH₃)₃ —(CH₂)₅CH₃ 65 —H —H —OCH(CH₃)₂ —C(CH₃)₃ —(CH₂)₄CH₃ 66 —H —H —OCH(CH₃)₂ —(CH₂)₃CH₃ —(CH₂)₅CH₃ 67 —H —H —OCH(CH₃)₂ —(CH₂)₅CH₃ —(CH₂)₅CH₃

TABLE 5

Ex- am- ple R¹ R² R³ —R⁶—R⁵— 68 —CH₃ —H —CH₃ —C(CH₃)₂(CH₂)₂— 69 —CH₃ —H —CH₃ —CH₂C(CH₃)₂(CH₂)₂— 70 —CH₃ —H —CH₃ —C(CH₃)₂CH₂OCO(CH₂)₃— 71 —CH₃ —H —CH₃ —C(CH₃)₂CH₂OCOC(CH₃)₂(CH₂)₃—

TABLE 6

Ex- am- ple R¹ R² R³ R⁷ R⁵ 72 —H —H —CH₃ —C(CH₃)₃ —(CH₂)₅CH₃ 73 —CH₃ —H —CH₃

—(CH₂)₅CH₃ 74 —OCH₃ —H —OCH₃ —C(CH₃)₃ —(CH₂)₃CH₃ 75 —CH₃ —H —CH₃ —C(CH₃)₃ —(CH₂)₃CH₃ 76 —CH₃ —H —CH₃

—(CH₂)₃CH₃ 77 —CH₃ —H —CH₃ —C(CH₃)₃ —(CH₂)₇CH₃ 78 —CH₃ —H —CH₃ —C(CH₃)₃ —CH₂CH₃ 79 —CH₃ —H —CH₃

—CH₂CH₃ 80 —CH₃ —H —CH₃ —C(CH₂)₅CH₃ —(CH₂)₅CH₃ 81 —H —H —CH₃ —CH₃ —(CH₂)₅CH₃ 82 —H —H —CH₃ —(CH₂)₃CH₃ —(CH₂)₅CH₃ 83 —H —H —CH₃ —(CH₂)₅CH₃ —(CH₂)₅CH₃ 84 —H —H —CH₃ —CH₃ —(CH₂)₇CH₃ 85 —H —H —CH₃ —(CH₂)₃CH₃ —(CH₂)₇CH₃ 86 —H —H —CH₃ —(CH₂)₅CH₃ —(CH₂)₇CH₃ 87 —H —H —OCH₃ —CH₃ —CH₂CH₃ 88 —H —H —OCH₃ —(CH₂)₃CH₃ —CH₂CH₃ 89 —H —H —OCH₃ —(CH₂)₅CH₃ —CH₂CH₃ 90 —H —H —OCH₃ —CH₃ —(CH₂)₃CH₃ 91 —H —H —OCH₃ —(CH₂)₃CH₃ —(CH₂)₃CH₃ 92 —H —H —OCH₃ —(CH₂)₅CH₃ —(CH₂)₃CH₃

TABLE 7

Example R¹ R³ R⁴ R⁷ R⁵ 93 —H —CH₃ —H —C(CH₃)₃ —(CH₂)₅CH₃ 94 —CH₃ —CH₃ —H —(CH₃)₂(CH₂)₃CH₃ —(CH₂)₅CH₃ 95 —OCH₃ —OCH₃ —H —C(CH₃)₃ —(CH₂)₅CH₃ 96 —CH₃ —CH₃ —H —C(CH₃)₃ —(CH₂)₃CH₃ 97 —CH₃ —CH₃ —H —C(CH₃)₂(CH₂)₃CH₃ —(CH₂)₃CH₃ 98 —CH₃ —CH₃ —H —C(CH₃)₂(CH₂)₅CH₃ —(CH₂)₃CH₃ 99 —CH₃ —CH₃ —H —C(CH₃)₃ —(CH₂)₇CH₃ 100  —CH₃ —CH₃ —H —C(CH₃)₃ —CH₂CH₃ 101  —CH₃ —CH₃ —H —C(CH₃)₂(CH₂)₅CH₃ —CH₂CH₃ 102  —CH₃ —CH₃ —H —(CH₂)₃CH₃ —(CH₂)₅CH₃ 103  —CH₃ —CH₃ —H —(CH₂)₅CH₃ —(CH₂)₅CH₃

TABLE 8

Example R¹ R³ R⁴ R⁶ R⁵ 104 —H —OCH₃ —H —CH₃ —CH₂CH₃ 105 —H —OCH₃ —H —(CH₂)₃CH₃ —CH₂CH₃ 106 —H —OCH₃ —H —(CH₂)₅CH₃ —CH₂CH₃ 107 —H —OCH₃ —H —CH₃ —(CH₂)₃CH₃ 108 —H —OCH₃ —H —(CH₂)₃CH₃ —(CH₂)₃CH₃

TABLE 9

Example R¹ R² R³ R⁶ R⁵ 109 —CH₃ —H —CH₃ —C(CH₃)₃ —(CH₂)₅CH₃ 110 —H —H —CH₃ —C(CH₃)₃ —(CH₂)₅CH₃ 111 —CH₃ —H —CH₃ —C(CH₃)₂(CH₂)₃CH₃ —(CH₂)₅CH₃ 112 —OCH₃ —H —OCH₃ —C(CH₃)₃ —(CH₂)₅CH₃ 113 —CH₃ —H —CH₃ —C(CH₃)₃ —(CH₂)₃CH₃ 114 —CH₃ —H —CH₃ —C(CH₃)₂(CH₂)₃CH₃ —(CH₂)₃CH₃ 115 —CH₃ —H —CH₃ —C(CH₃)₂(CH₂)₅CH₃ —(CH₂)₃CH₃ 116 —CH₃ —H —CH₃ —C(CH₃)₃ —(CH₂)₇CH₃ 117 —CH₃ —H —CH₃ —C(CH₃)₃ —CH₂CH₃ 118 —CH₃ —H —CH₃ —C(CH₃)₂(CH₂)₅CH₃ —CH₂CH₃

EXPERIMENTAL EXAMPLE 1

ACAT Inhibitory Activity

A high cholesterol feed [a feed added with cholesterol (1%), Clea Japan, Inc.] was fed to male Japanese white rabbits weighing 2-2.5 kg at 100 g per day and the rabbits were bred for 4 weeks. The rabbits were killed by bleeding under anesthesia and small intestine was removed. The mucous membrane of small intestine was peeled, collected and homogenated. The homogenate was centrifuged at 4° C. and 10,000 rpm for 15 min. The obtained supernatant was further centrifuged at 4° C. and 41,000 rpm for 30 minutes to give microsomal fractions. Using this microsomal suspension as an enzyme sample, dimethyl sulfoxide (DMSO, 5 μl) or a test compound dissolved in DMSO (test compound solution, 5 μl), and a reaction substrate [1-¹⁴C]-oleoyl CoA were added to a reaction buffer. After incubation at 37° C. for 5 minutes, a chloroform-methanol mixture was added to stop the reaction. Water was added thereto and mixed, and chloroform layer was separated. The solvent was evaporated to dryness, and the residue was dissolved in hexane. The mixture was subjected to thin layer chromatography using a silica gel plate. The spots of cholesteryl oleate on the silica gel plate were scraped, and quantitatively assayed on a liquid scintillation counter. The ACAT inhibitory activity of the test compound was expressed as a proportion (%) of inhibition of cholesteryl oleate, namely, the proportion of inhibition of cholesteryl oleate production as compared to control.

The results are shown in Tables 10-11.

EXPERIMENTAL EXAMPLE 2

Serum Total Cholesterol Reducing Effect

Male Wister rats weighing 180-200 g were bred under free access to a high cholesterol feed [added with cholesterol (1%), cholic acid (0.5%) and coconut oil (10%), Clea Japan, Inc.] for 3 days, during which period a test compound (10-100 mg/kg) suspended in 5% gum arabic solution was forcibly administered once a day orally for 3 days. Only 5% gum arabic solution was administered to control animals. After final administration, the test animals were fasted for 5 hours and the blood was taken. The serum total cholesterol level was determined using a commercially available assay kit (cholesterol-E-Test Wako, Wako Pure Chemical Industries, Ltd.). The activity of the test compound was expressed as a proportion (%) of reduction of serum total cholesterol level, namely, the proportion of reduction of serum total cholesterol as compared to control.

The results are shown in Tables 10-11.

EXPERIMENTAL EXAMPLE 3

LDL Peroxidation Inhibitory Effect

Male Japanese white rabbits weighing 2-2.5 kg were bred on 100 g per day of a high cholesterol feed [added with cholesterol (1%), Clea Japan, Inc.] for 4 weeks. The blood was taken from carotid and plasma was obtained. Then, LDL was fractionated from the plasma by ultracentrifugation, dialyzed for one day and preserved at 4° C. LDL (400 μg) and aqueous copper sulfate solution (final concentration 5 μM) were added to bufferized Ham F-10 medium (2 ml, GIBCO, USA). DMSO or a solution (20 μl) of test compound dissolved in DMSO was added and the mixture was incubated at 37° C. for 24 hours. After the completion of the incubation, LDL peroxide in medium was allowed to develop color by thiobarbituric acid method and assayed as malondialdehyde. The activity of the test compound was expressed as malondialdehyde production inhibitory ratio (%), namely, the proportion of inhibition of production of malondialdehyde as compared to control.

The results are shown in Tables 10-11.

EXPERIMENTAL EXAMPLE 4

Plasma Lipoperoxidation Inhibitory Effect

The blood was taken from male Japanese white rabbits weighing 2-2.5 kg under anesthesia and heparinized plasma was separated by a conventional method. To the plasma (2.0 ml) was added DMSO or a solution (20 μl, final concentration 10⁻⁵ M) of test compound dissolved in DMSO, and aqueous copper sulfate solution (final concentration 5 mM) was added immediately thereafter. The mixture was incubated at 37° C. for 3 hours. After the completion of the incubation, 20% trichloroacetic acid was added to stop the reaction. Then, the mixture was centrifuged at 4° C., 4,500 rpm for 15 minutes. The lipoperoxide in the supernatant thus obtained was assayed as malondialdehyde upon color development by thiobarbituric acid method. The activity of the test compound was expressed as malondialdehyde production inhibitory ratio (%), namely, the proportion of inhibition of production of malondialdehyde as compared to control.

The results are shown in Tables 10-11.

TABLE 10 Result of Result of Result of Result of Exp. Ex. 1 Exp. Ex. 2 Exp. Ex. 3 Exp. Ex. 4 Test compound (%) *1 (%) *2 (%) *3 (%) *4 Example 1  76.6 **  12.9 *** 99.2 94.3 Example 2  59.9 ** — 99.5 95.8 Example 3  97.5 ** 54.4 ** 93.3 90.4 Example 4  94.9 ** 21.4 ** 59.5 92.0 Example 6  99.8 ** 53.3 ** 24.8 37.4 Example 7  96.2 ** 19.2 ** 86.6 86.4 Example 8  99.7 ** 48.0 ** 91.9 85.1 Example 9  81.3 ** 51.3 ** 93.7 81.6 Example 10 98.7 ** 55.2 ** 18.3 82.6 Example 11 99.2 ** 59.5 *  91.6 78.8 Example 12 71.9 ** 30.3 ** 90.3 74.9 Example 13 96.0 ** 28.4 ** 82.4 87.1 Example 14 96.6 ** 53.9 ** 88.3 93.1 Example 15 93.1 ** 35.0 *  12.3 90.1 Example 17 96.7 ** 27.0 *  89.6 91.1 Example 18 69.6 *  22.3 ** 34.0 87.1 Example 20 22.7 *  7.8 * 67.8 91.1 Example 21 94.4 ** 28.3 *  92.1 91.4 Example 22 78.5 **  38.6 *** 40.6 92.8 Example 23 88.8 ** 23.6 ** 54.8 90.5 *1 ACAT inhibition (concentration *: 10⁻⁴M, **: 10⁻⁵M) *2 reduction of serum total cholesterol (dose *: 10 mg/kg/day, **: 30 mg/kg/day, ***: 100 mg/kg/day) *3 LDL peroxidation inhibition (concentration: 10⁻⁵M) *4 plasma lipoperoxidation inhibition (concentration: 10⁻⁵M)

TABLE 11 Result of Result of Result of Result of Exp. Ex. 1 Exp. Ex. 2 Exp. Ex. 3 Exp. Ex. 4 Test compound (%) *1 (%) *2 (%) *3 (%) *4 Example 24 97.1 ** 28.3 ** 42.2 90.5 Example 25 96.8 ** 50.5 ** 92.2 93.1 Example 26 26.5 ** 37.4 ** 43.3 90.1 Example 27 95.5 **  56.9 *** 92.2 91.1 Example 28 79.4 **  20.8 *** 92.2 91.1 Example 29 86.6 **  6.9 ** 91.9 91.1 Example 30 83.0 **  25.7 *** 94.1 91.8 Example 31 93.8 ** 49.0 ** 90.3 90.8 Example 32 93.9 ** 57.4 ** 85.3 89.1 Example 33 82.2 **  7.2 ** 87.0 89.5 Example 34 91.0 ** 50.2 ** 82.9 89.8 Example 35 84.6 ** 20.8 ** 71.9 91.4 Example 36 95.9 ** 50.2 ** 83.2 89.1 YM-750 92.3 ** 43.8 ** 0  — Probucol  3.4 ** 7.3 * 89.4 87.5 *1 ACAT inhibition (concentration *: 10⁻⁴M, **: 10⁻⁵M) *2 reduction of serum total cholesterol (dose *: 10 mg/kg/day, **: 30 mg/kg/day, ***: 100 mg/kg/day) *3 LDL peroxidation inhibition (concentration: 10⁻⁵M) *4 plasma lipoperoxidation inhibition (concentration: 10⁻⁵M) YM-750: 1-cycloheptyl-1-[(2-fluorenyl)methyl]-3-(2,4,6-trimethylphenyl)urea Probucol: 4,4′-isopropylidenedithiobis(2,6-di-t-butylphenol)

FORMULATION EXAMPLE 1

Tablets having the following composition are prepared by a conventional method.

Compound of Example 3 25 mg Polyvinylpyrrolidone 20 mg Starch 75 mg Magnesium stearate  2 mg

FORMULATION EXAMPLE 2

Capsules having the following composition are prepared by a conventional capsule packing method.

Compound of Example 6 100 mg Lactose  25 mg Magnesium stearate  1 mg

The heterocyclic derivative and pharmaceutically acceptable salts thereof of the present invention show superior ACAT inhibitory activity and lipoperoxidation inhibitory activity, and are useful as ACAT inhibitors or hyperlipemia inhibitors. To be specific, they are useful for the prevention and treatment of arteriosclerotic lesions such as arteriosclerosis, hyperlipemia and diabetes, as well as ischemic diseases of brain, heart and the like. 

What is claimed is:
 1. A method of preparing a compound of the formula

wherein R¹ and R³ are methyl, R² is hydrogen, R⁴ is —NHCO—R⁶, R⁶ is selected from the group consisting of C₁-C₁₂ alkyl, cyclo C₃-C₆ alkyl, cyclo C₃-C₆ alkyl C₁-C₃ alkyl, phenyl, naphthyl, phenyl C₁-C₄ alkyl, naphthyl C₁-C₄ alkyl, pyrrolidinyl, piperidyl, piperidino, morpholinyl, morpholino, piperazinyl, pyrrolyl, imidazolyl, pyridyl, pyrrolidinyl C₁-C₈ alkyl, piperidyl C₁-C₈ alkyl, piperidino C₁-C₈ alkyl, morpholinyl C₁-C₈ alkyl, morpholino C₁-C₈ alkyl, piperazinyl C₁-C₈ alkyl, pyrrolyl C₁-C₈ alkyl, imidazolyl C₁-C₈ alkyl, pyridyl C₁-C₈ alkyl (wherein any of the foregoing is unsubstituted or substituted with a C₁-C₄ alkyl, amino, hydroxy, di(C₁-C₄)alkylamino, C₁-C₄ aminoalkyl, C₁-C₄ alkoxy, carboxyl, C₁-C₄ alkoxycarbonyl, carboxy(C₁-C₄)alkyl, C₂-C₅ acyloxy, phenyl, phenoxy, halogen, or phenyl di(C₁-C₄)alkylamino), —R^(A)SO₃A, and —R^(B)PO₃B where R^(A) and R^(B) are each C₁-C₈ alkylene and A and B are each alkali metal or hydrogen atom, —NR⁷R⁸ where R⁷ is selected from the group consisting of C₁-C₁₂ alkyl, cyclo C₃-C₆ alkyl, cyclo C₃-C₆ alkyl C₁-C₃ alkyl, phenyl, naphthyl, phenyl C₁-C₄ alkyl, and naphthyl C₁-C₄ alkyl (wherein any of the foregoing is optionally substituted with a C₁-C₄ alkyl, amino, hydroxy, di(C₁-C₄)alkylamino, C₁-C₄ aminoalkyl, C₁-C₄ alkoxy, carboxyl, C₁-C₄ alkoxycarbonyl, carboxy(C₁-C₄)alkyl, C₂-C₅ acyloxy, phenyl, phenoxy, halogen, or phenyl di(C₁-C₄)alkylamino), and R⁸ is hydrogen atom or C₁-C₄ alkyl, or —R⁹—OCOR¹⁰ where R⁹ is C₁-C₈ alkylene and R¹⁰ is selected from the group consisting of C₁-C₁₂ alkyl, pyrrolidinyl, piperidyl, piperidino, morpholinyl, morpholino, piperazinyl, pyrrolyl, imidazolyl, pyridyl, pyrrolidinyl C₁-C₈ alkyl, piperidyl C₁-C₈ alkyl, piperidino C₁-C₈ alkyl, morpholinyl C₁-C₈ alkyl, morpholino C₁-C₈ alkyl, piperazinyl C₁-C₈ alkyl, pyrrolyl C₁-C₈ alkyl, imidazolyl C₁-C₈ alkyl, and pyridyl C₁-C₈ alkyl (wherein any of the foregoing is optionally substituted with a C₁-C₄ alkyl, amino, hydroxy, di(C₁-C₄)alkylamino, C₁-C₄ aminoalkyl, C₁-C₄ alkoxy, carboxyl, C₁-C₄ alkoxycarbonyl, carboxy(C₁-C₄)alkyl, C₂-C₅ acyloxy, phenyl, phenoxy, halogen, or phenyl di(C₁-C₄)alkylamino), R⁵ is selected from the group consisting of C₁-C₁₂ alkyl, cyclo C₃-C₆ alkyl, cyclo C₃-C₆ alkyl C₁-C₃ alkyl, phenyl, naphthyl, phenyl C₁-C₄ alkyl, naphthyl C₁-C₄ alkyl, pyrrolidinyl, piperidyl, piperidino, morpholinyl, morpholino, piperazinyl, pyrrolyl, imidazolyl, pyridyl, pyrrolidinyl C₁-C₈ alkyl, piperidyl C₁-C₈ alkyl, piperidino C₁-C₈ alkyl, morpholinyl C₁-C₈ alkyl, morpholino C₁-C₈ alkyl, piperazinyl C₁-C₈ alkyl, pyrrolyl C₁-C₈ alkyl, imidazolyl C₁-C₈ alkyl, pyridyl C₁-C₈ alkyl (wherein any of the foregoing is optionally substituted with a C₁-C₄ alkyl, amino, hydroxy, di(C₁-C₄)alkylamino, C₁-C₄ aminoalkyl, C₁-C₄ alkoxy, carboxyl, C₁-C₄ alkoxycarbonyl, carboxy(C₁-C₄)alkyl, C₂-C₅ acyloxy, phenyl, phenoxy, halogen, or phenyl di(C₁-C₄)alkylamino, C₂-C₈ alkenyl, C₂-C₈ alkynyl, di(C₁-C₈)alkylamino(C₂-C₅)acyloxy(C₁-C₈)alkyl, —R^(D)SO₃D, and —R^(E)PO₃E where R^(D) and R^(E) are each C₁-C₈ alkylene and D and E are each alkali metal or hydrogen atom, and m is 1, said method comprising: providing 1-acetyl-5-bromo-4,6-dimethylindoline; introducing a nitro substituent to the 7- position of said 1-acetyl-5-bromo-4,6-dimethylidoline; reducing said nitro substituent to an amino substituent; converting said amino substituent to an amide group of the formula R⁶C(O)—, wherein R⁶ is as defined above; reductively removing the bromine atom at the 5-position; and removing the acetyl substituent at the 1- position and thereafter covalently bonding R⁵ to the indoline nitrogen at the 1-position.
 2. The method of claim 1, wherein said amino substituent is converted to an amide group of the formula R⁶C(O)— by reacting said amino substituent with an acid of the formula R⁶CO₂H or an acid halide of the formula R⁶COX, wherein X is halogen atom.
 3. The method of claim 2, wherein X is chloride.
 4. The method of claim 1, wherein said amino substituent is converted to an amide group of the formula R⁶C(O)— by converting said amino substituent to an isocyanate and reacting said isocyanate with an amine of the formula HNR⁷R⁸.
 5. The method of claim 1, wherein said amino substituent is converted to an amide group of the formula R⁶C(O)— by reacting the amino substituent at the 7-position with an isocyanate of the formula R⁷—NCO.
 6. The method of claim 1, wherein said acetyl is removed via base hydrolysis.
 7. The method of claim 6, wherein said base is hydroxide ion.
 8. The method of claim 1, wherein said R⁵ is covalently bonded to said indoline nitrogen by reacting said indoline nitrogen with a compound of the formula R⁵X, wherein X is halogen atom.
 9. The method of claim 8, wherein R⁵ is unsubstituted.
 10. The method of claim 8, wherein R⁵ is substituted with alkyl, amino, hydroxy, dialkylamino, aminoalkyl, alkxoy, carboxyl, alkoxycarbonyl, carboxyalkyl, acyloxy, phenyl, phenoxy or halogen.
 11. The method of claim 1, wherein said bromine atom at the 5-position is reductively removed in the presence of a catalyst.
 12. The method of claim 11, wherein said catalyst is palladium.
 13. The method of claim 1, wherein said nitro substituent is reduced to an amino substituent, and said bromine atom at the 5-position is reductively removed, in one step.
 14. The method of claim 13, wherein said amino substituent is converted to an amide group of the formula R⁶C(O)— by reacting the amino substituent at the 7-position with an acid halide of the formula R⁶COX, wherein X is a halogen atom.
 15. The method of claim 14, wherein said amino substituent is converted to an amide group of the formula R⁶C(O)— by converting the amino substituent at the 7-position to an isocyanate and reacting said isocyanate with an amine of the formula HNR⁷R⁸.
 16. A method of preparing a compound of the formula

wherein R¹ and R³ are methyl, R² is hydrogen, R⁴ is —NHCO—R⁶, R⁵ is acetyl, R⁶ is selected from the group consisting of C₁-C₁₂ alkyl, cyclo C₃-C₆ alkyl, cyclo C₃-C₆ alkyl C₁-C₃ alkyl, phenyl, naphthyl, phenyl C₁-C₄ alkyl, naphthyl C₁-C₄ alkyl, pyrrolidinyl, piperidyl, piperidino, morpholinyl, morpholino, piperazinyl, pyrrolyl, imidazolyl, pyridyl, pyrrolidinyl C₁-C₈ alkyl, piperidyl C₁-C₈ alkyl, piperidino C₁-C₈ alkyl, morpholinyl C₁-C₈ alkyl, morpholino C₁-C₈ alkyl, piperazinyl C₁-C₈ alkyl, pyrrolyl C₁-C₈ alkyl, imidazolyl C₁-C₈ alkyl, pyridyl C₁-C₈ alkyl (wherein any of the foregoing is unsubstituted or substituted with a C₁-C₄ alkyl, amino, hydroxy, di(C₁-C₄)alkylamino, C₁-C₄ aminoalkyl, C₁-C₄ alkoxy, carboxyl, C₁-C₄ alkoxycarbonyl, carboxy(C₁-C₄)alkyl, C₂-C₅ acyloxy, phenyl, phenoxy, halogen, or phenyl di(C₁-C₄)alkylamino), —R^(A)SO₃A, and —R^(B)PO₃B where R^(A) and R^(B) are each C₁-C₈ alkylene and A and B are each alkali metal or hydrogen atom, —NR⁷R⁸ where R⁷ is selected from the group consisting of C₁-C₁₂ alkyl, cyclo C₃-C₆ alkyl, cyclo C₃-C₆ alkyl C₁-C₃ alkyl, phenyl, naphthyl, phenyl C₁-C₄ alkyl, and naphthyl C₁-C₄ alkyl (wherein any of the foregoing is optionally substituted with a C₁-C₄ alkyl, amino, hydroxy, di(C₁-C₄)alkylamino, C₁-C₄ aminoalkyl, C₁-C₄ alkoxy, carboxyl, C₁-C₄ alkoxycarbonyl, carboxy(C₁-C₄)alkyl, C₂-C₅ acyloxy, phenyl, phenoxy, halogen, or phenyl di(C₁-C₄)alkylamino), and R⁸ is hydrogen atom or C₁-C₄ alkyl, or —R⁹—OCOR¹⁰ where R⁹ is C₁-C₈ alkylene and R¹⁰ is selected from the group consisting of C₁-C₁₂ alkyl, pyrrolidinyl, piperidyl, piperidino, morpholinyl, morpholino, piperazinyl, pyrrolyl, imidazolyl, pyridyl, pyrrolidinyl C₁-C₈ alkyl, piperidyl C₁-C₈ alkyl, piperidino C₁-C₈ alkyl, morpholinyl C₁-C₈ alkyl, morpholino C₁-C₈ alkyl, piperazinyl C₁-C₈ alkyl, pyrrolyl C₁-C₈ alkyl, imidazolyl C₁-C₈ alkyl, and pyridyl C₁-C₈ alkyl (wherein any of the foregoing is optionally subtituted with a C₁-C₄ alkyl, amino, hydroxy, di(C₁-C₄)alkylamino, C₁-C₄ aminoalkyl, C₁-C₄ alkoxy, carboxyl, C₁-C₄ alkoxycarbonyl, carboxy(C₁-C₄)alkyl, C₂-C₅ acyloxy, phenyl, phenoxy, halogen, or phenyl di(C₁-C₄)alkylamino), and M is 1, said method comprising: providing 1-acetyl-5-bromo-4,6-dimethylindoline; introducing a nitro substituent to the 7-position of said 1-acetyl-5-bromo-4,6-dimethylindoline; reducing said nitro substituent to an amino substituent; converting said amino substituent to an amide group of the formula R⁶C(O)—, wherein R⁶ is as defined above; and reductively removing the bromine atom at the 5-position. 